The output of both approaches is an amplified template,[url=http://www.bulksgo.com/?kw=spacer-beads]wholesale spacer beads[/url], either as many 'clonal' copies of a single plasmid insert present within a spatially isolated bacterial colony that can be picked, or as many PCR amplicons present within a single reaction volume.
The sequencing biochemistry takes place in a 'cycle sequencing' reaction, in which cycles of template denaturation, primer annealing and primer extension are performed.
The primer is complementary to known sequence immediately flanking the region of interest.
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